Fluorimetric Determination of Peroxynitrite Based on an Enzymatic Reaction
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چکیده
منابع مشابه
Fluorimetric determination of peroxynitrite based on an enzymatic reaction.
A novel fluorimetric method for the determination of peroxynitrite (ONOO-) using hemoglobin (Hb) as a catalyst is described. The method employs the reaction of ONOO with thiamine (TM), a colorless, non-fluorescent reagent in a glycine-NaCl-NaOH buffer solution (pH 12.7), to generate a highly fluorescent product, thiochrome (TC). The fluorescent product was monitored by fluorimetry. A linear cal...
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A simple spectrofluorimetric analysis of a local anaesthetic named procaine using a specific labelling reagent for primary amino groups, has been developed. Because procaine shows very weak native fluorescence, the technique of spectrofluorimetry has been very much limited for its determination. A detail study of the variables affecting the derivatisation reaction (pH, fluorescamine (FC) concen...
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STRONGLY ALKALINE SOLUTIONS ninhydrin (1,2,3 indantrione hy) combines with guanidine, monosubstituted guanidines, and disubstituted guanidines to form highly fluorescent addition ucts (1). This reaction has been used as the basis for a simple, itive fluorimetric method for the determination of creatine in m, blood, and urine. he most widely used methods for determining creatine are based n the ...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2005
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.21.445